Tsolwana Nature Reserve is a nature reserve in Eastern Cape Province, South Africa that is managed by Eastern Cape Parks.
It is located along the northern slopes of the Winterberg Mountains at the edge of Karoo. Its western boundary is formed by the upper Black Kei River. It is situated between Cradock and Queenstown and near Tarkastad.
The reserve's name originates from the Xhosa word for "spike", due to the presence of the cone-shaped hill, Spitskop.
The reserve is mostly made up of grassy plains and acacia thornveld. The reserve is home to many animals, including the white rhino, giraffe, eland, blesbok, mountain reedbuck, Cape Mountain zebra, and springbok. This variety of animals makes it a poular site for game viewing.